تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Evaluation of the Effects of Airborne Particulate Matter on Bone Marrow-Mesenchymal Stem Cells (BM-MSCs): Cellular, Molecular and Systems Biological Approaches
Evaluation of the Effects of Airborne Particulate Matter on Bone Marrow-Mesenchymal Stem Cells (BM-MSCs): Cellular, Molecular and Systems Biological Approaches
 
لغة الوثيقة : الانجليزية 
المستخلص : Particulate matter (PM) contains heavy metals that affect various cellular functions and gene expression associated with a range of acute and chronic diseases in humans. However, the specific effects they exert on the stem cells remain unclear. Here, we report the effects of PM collected from the city of Jeddah on proliferation, cell death, related gene expression and systems of biological analysis in bone marrow mesenchymal stem cells (BM-MSCs), with the aim of understanding the underlying mechanisms. PM2.5 and PM10 were tested in vitro at various concentrations (15 to 300 µg/mL) and durations (24 to 72 h). PMs induced cellular stress including membrane damage, shrinkage and death. Lower concentrations of PM2.5 increased proliferation of BM-MSCs, while higher concentrations served to decrease it. PM10 decreased BM-MSCs proliferation in a concentration-dependent manner. The X-ray fluorescence spectrometric analysis showed that PM contains high levels of heavy metals. Ingenuity Pathway Analysis (IPA) and hierarchical clustering analyses demonstrated that heavy metals were associated with signaling pathways involving cell stress/death, cancer and chronic diseases. qRT-PCR results showed differential expression of the apoptosis genes (BCL2, BAX); inflammation associated genes (TNF-α and IL-6) and the cell cycle regulation gene (p53). We conclude that PM causes inflammation and cell death, and thereby predisposes to chronic debilitating diseases. 
ردمد : 1319-0989 
اسم الدورية : مجلة الآداب و العلوم الإنسانية 
المجلد : 14 
العدد : 4 
سنة النشر : 1438 هـ
2017 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Sunday, July 9, 2017 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Muhammad Abu-ElmagdAbu-Elmagd, Muhammad باحث رئيسيدكتوراهmabuelmagd@kau.edu.sa
Mansour A. AlghamdiAlghamdi, Mansour A.باحثدكتوراهmans99@gmail.com
Magdy ShamyShamy, Magdy باحثدكتوراهmshamy@kau.edu.sa
Mamdouh I. KhoderKhoder, Mamdouh I.باحثدكتوراهkhoder_55@yahoo.com
Max CostaCosta, Max باحثدكتوراهmax.costa@nyumc.org
Mourad AssidiAssidi, Mourad باحثدكتوراهmourad.assidi@gmail.com
Roaa KadamKadam, Roaa باحثماجستيرrkadem@kau.edu.sa
Haneen AlsehliAlsehli, Haneen باحثماجستيرhalsehli@kau.edu.sa
Mamdooh GariGari, Mamdooh باحثدكتوراهmgari@kau.edu.sa
Peter Natesan PushparajPushparaj, Peter Natesanباحثدكتوراهpeter.n.pushparaj@gmail.com
Gauthaman KalamegamKalamegam, Gauthaman باحثدكتوراهkgauthaman@kau.edu.sa
Mohammed H. Al-QahtaniAl-Qahtani, Mohammed H.باحثدكتوراهmhalqahtani@kau.edu.sa

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