تفاصيل الوثيقة

نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Improved Y-STR typing for disaster victim identification, missing persons investigations, and historical human skeletal remains
Improved Y-STR typing for disaster victim identification, missing persons investigations, and historical human skeletal remains
 
لغة الوثيقة : الانجليزية 
المستخلص : Bones are a valuable source of DNA in forensic, anthropological, and archaeological investigations. There are a number of scenarios in which the only samples available for testing are highly degraded and/or skeletonized. Often it is necessary to perform more than one type of marker analysis on such samples in order to compile sufficient data for identification. Lineage markers, such as Y-STRs and mitochondrial DNA (mtDNA), represent important systems to complement autosomal DNA markers and anthropological metadata in making associations between unidentified remains and living relatives or for characterization of the remains for historical and archaeological studies. In this comparative study, Y-STR typing with both Yfiler™ and Yfiler™ Plus (Thermo Fisher Scientific, Waltham, MA, USA) was performed on a variety of human skeletal remains, including samples from the American Civil War (1861–1865), the late nineteenth century gold rush era in Deadwood, SD, USA (1874–1877), the Seven Years’ War (1756–1763), a seventeenth-century archaeological site in Raspenava, Bohemia (Czech Republic), and World War II (1939–1945). The skeletal remains used for this study were recovered from a wide range of environmental conditions and were extracted using several common methods. Regardless of the DNA extraction method used and the age/condition of the remains, 22 out of 24 bone samples yielded a greater number of alleles using the Yfiler™ Plus kit compared to the Yfiler™ kit using the same quantity of input DNA. There was no discernable correlation with the degradation index values for these samples. Overall, the efficacy of the Yfiler™ Plus assay was demonstrated on degraded DNA from skeletal remains. Yfiler™ Plus increases the discriminatory power over the previous generation multiplex due to the larger set of Y-STR markers available for analysis and buffer modifications with the newer version kit. Increased haplotype resolution is provided to infer or refute putative genetic relationships. 
ردمد : 1437-1596 
اسم الدورية : International Journal of Legal Medicine 
المجلد : 132 
العدد : 6 
سنة النشر : 1439 هـ
2018 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Tuesday, June 25, 2019 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Angie AmbersAmbers, Angie باحث رئيسيدكتوراهangie.ambers2@unthsc.edu
Jitka VotrubovaVotrubova, Jitka باحثدكتوراه 
Daniel VanekVanek, Daniel باحثدكتوراه 
Antti SajantilaSajantila, Antti باحثدكتوراه 
Bruce BudowleBudowle, Bruce باحثدكتوراه 

الملفات

اسم الملفالنوعالوصف
 44530.pdf pdf 

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