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Document Type |
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Article In Journal |
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Document Title |
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A Qualitative and Quantitative Assay to Study DNA/Drug Interaction based on the Sequence Selective Inhibition of Restriction Endonucleases الفحص النوعي والكمي لدراسة الحمض النووي / التفاعل المخدرات استنادا إلى تثبيط تسلسل الانتقائي للنوكليازات الاقتطاع الداخلية |
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Subject |
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BIOINFORMATICS AND BIOTECHNOLOGY |
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Document Language |
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English |
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Abstract |
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Purpose: To explore the use of restriction inhibition assay (RIA) to study the binding specificity of some anticancer drugs.
Methods: A 448 bp DNA fragment derived from pBCKS+ plasmid (harboring the polylinker region with multiple restriction endonuclease sites) was used as a template for sequence selective inhibition of the test drugs. The template was incubated with different concentrations of anticancer drugs (adriamycin, daunomycin, mitoxantrone, distamycin-A, berberine and palmatine) prior to digestion with restriction endonucleases - HindIII, EcoRI and EcoRV.
Results: Mitoxantrone, adriamycin and daunomycin showed specificity for HindIII restriction site (5’-AAGCTT-3’) at 220, 100 and 100 μM concentration, respectively. Conversely, distamycin-A showed an affinity for EcoRI (5’-AAATGC-3’) restriction sites at a concentration of 10 μM. No binding was observed for berberine and palmatine at a maximum concentration of 2 mM at HindIII, EcoRI and EcoRV restriction sites, respectively.
Conclusion: The inhibition of endonucleases by mitoxantrone, adriamycin, daunomycin, distamycin-A, provides direct evidence of the co-existence of concentration and sequence specificity for drug-DNA interaction as well as the need to explore the possible use of RIA for demonstrating the binding specificity of anticancer drugs. |
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ISSN |
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1596-5996 |
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Journal Name |
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TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH |
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Volume |
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11 |
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Issue Number |
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5 |
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Publishing Year |
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1434 AH
2012 AD |
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Article Type |
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Article |
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Added Date |
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Wednesday, November 13, 2013 |
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Researchers
| عاصف سيد | Syed, Asif | Researcher | Doctorate | asif_srmcbt@yahoo.com |
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